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mouse phospho stat6  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc mouse phospho stat6
    Mouse Phospho Stat6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+stat6+tyr641/Phospho-Stat6+(Tyr641)+Rabbit+mAb/pmc13049607-338-66-69
    Average 96 stars, based on 233 article reviews
    mouse phospho stat6 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Tubule-derived CCN1 drives renal repair via α v β 5 -STAT6-ARG1-dependent reprogramming of macrophages.
    Article Snippet: Equal amounts of protein (30-50 μg) were separated by SDSPAGE and transferred onto PVDF membranes (Millipore, Burlington, MA, USA). .. Membranes were blocked with 5% non-fat milk or 5% BSA for 1 h at room temperature and incubated overnight at 4°C with CCN1 (#39382), ARG1 (#93668), Phospho-STAT6 (Tyr641) (#9361), STAT6 (#5397) and GAPDH (#2118) primary antibodies (all 1:1000, Cell Signaling Technology). ..

    Article Title: Tubule-derived CCN1 drives renal repair via α v β 5 -STAT6-ARG1-dependent reprogramming of macrophages
    Article Snippet: Equal amounts of protein (30–50 μg) were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, Burlington, MA, USA). .. Membranes were blocked with 5% non-fat milk or 5% BSA for 1 h at room temperature and incubated overnight at 4°C with CCN1 (#39382), ARG1 (#93668), Phospho-STAT6 (Tyr641) (#9361), STAT6 (#5397) and GAPDH (#2118) primary antibodies (all 1:1000, Cell Signaling Technology). ..

    Article Title: Malaria pigment hemozoin drives M1 pro-inflammatory macrophage polarization in vitro
    Article Snippet: Membranes were blocked for 1 h at room temperature with a solution containing 5% nonfat dried milk powder (EuroClone) in TBS-T (0.01% Tween 20 in TBS), and subsequently incubated overnight at 4 °C with primary antibodies (Ab) diluted in the same blocking solution. .. The primary Abs used included total STAT6 (1:1000 dilution; Cell Signaling Technology, #9362) and phospho-STAT6 (Tyr641) (1:500 dilution; Cell Signaling Technology, #9361). β-actin was used as a loading control, using the anti-β-actin monoclonal Ab (mAb) 13E5 (1:1000 dilution; Cell Signaling Technology, #4970) prepared in 5% nonfat dried milk powder in TBS-T. After three washes with TBS-T, membranes were incubated for 1 h at room temperature with a horseradish peroxidase (HRP)-linked anti-rabbit IgG secondary Ab (1:2000 dilution; Cell Signaling Technology, #7074) diluted in 5% nonfat dried milk powder in TBS-T. .. Protein bands were visualized using an enhanced chemiluminescence detection kit (Amersham GE Healthcare, Amersham, UK), performed according to the manufacturer’s instructions.

    Article Title: Malaria pigment hemozoin drives M1 pro-inflammatory macrophage polarization in vitro.
    Article Snippet: Membranes were blocked for 1h at room temperature with a solution 532 containing 5% nonfat dried milk powder (EuroClone) in TBS-T (0.01% Tween 20 in TBS), and 533 subsequently incubated overnight at 4°C with primary antibodies (Ab) diluted in the same blocking 534 solution. .. The primary Abs used included total STAT6 (1:1,000 dilution; Cell Signaling Technology, 535 #9362) and phospho-STAT6 (Tyr641) (1:500 dilution; Cell Signaling Technology, #9361). β-actin 536 was used as a loading control, using the anti-β-actin monoclonal Ab (mAb) 13E5 (1:1,000 dilution; 537 Cell Signaling Technology, #4970) prepared in 5% nonfat dried milk powder in TBS-T. After three 538 washes with TBS-T, membranes were incubated for 1h at room temperature with a horseradish 539 peroxidase (HRP)-linked anti-rabbit IgG secondary Ab (1:2,000 dilution; Cell Signaling Technology, 540 #7074) diluted in 5% nonfat dried milk powder in TBS-T. .. Protein bands were visualized using an 541 enhanced chemiluminescence detection kit (Amersham GE Healthcare, Amersham, UK), 542 performed according to the manufacturer’s instructions.

    Control:

    Article Title: Malaria pigment hemozoin drives M1 pro-inflammatory macrophage polarization in vitro
    Article Snippet: Membranes were blocked for 1 h at room temperature with a solution containing 5% nonfat dried milk powder (EuroClone) in TBS-T (0.01% Tween 20 in TBS), and subsequently incubated overnight at 4 °C with primary antibodies (Ab) diluted in the same blocking solution. .. The primary Abs used included total STAT6 (1:1000 dilution; Cell Signaling Technology, #9362) and phospho-STAT6 (Tyr641) (1:500 dilution; Cell Signaling Technology, #9361). β-actin was used as a loading control, using the anti-β-actin monoclonal Ab (mAb) 13E5 (1:1000 dilution; Cell Signaling Technology, #4970) prepared in 5% nonfat dried milk powder in TBS-T. After three washes with TBS-T, membranes were incubated for 1 h at room temperature with a horseradish peroxidase (HRP)-linked anti-rabbit IgG secondary Ab (1:2000 dilution; Cell Signaling Technology, #7074) diluted in 5% nonfat dried milk powder in TBS-T. .. Protein bands were visualized using an enhanced chemiluminescence detection kit (Amersham GE Healthcare, Amersham, UK), performed according to the manufacturer’s instructions.

    Article Title: Malaria pigment hemozoin drives M1 pro-inflammatory macrophage polarization in vitro.
    Article Snippet: Membranes were blocked for 1h at room temperature with a solution 532 containing 5% nonfat dried milk powder (EuroClone) in TBS-T (0.01% Tween 20 in TBS), and 533 subsequently incubated overnight at 4°C with primary antibodies (Ab) diluted in the same blocking 534 solution. .. The primary Abs used included total STAT6 (1:1,000 dilution; Cell Signaling Technology, 535 #9362) and phospho-STAT6 (Tyr641) (1:500 dilution; Cell Signaling Technology, #9361). β-actin 536 was used as a loading control, using the anti-β-actin monoclonal Ab (mAb) 13E5 (1:1,000 dilution; 537 Cell Signaling Technology, #4970) prepared in 5% nonfat dried milk powder in TBS-T. After three 538 washes with TBS-T, membranes were incubated for 1h at room temperature with a horseradish 539 peroxidase (HRP)-linked anti-rabbit IgG secondary Ab (1:2,000 dilution; Cell Signaling Technology, 540 #7074) diluted in 5% nonfat dried milk powder in TBS-T. .. Protein bands were visualized using an 541 enhanced chemiluminescence detection kit (Amersham GE Healthcare, Amersham, UK), 542 performed according to the manufacturer’s instructions.



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    HA from myofibroblasts induces macrophage M2 polarization via the <t>CD44/STAT6</t> axis. (A) RAW264.7 macrophages were stimulated with HA for 24 h, and flow cytometry was performed to assess the expression of CD206 (FITC) and CD86 (PE) (n=3 for each group). (B) The content of TGF-β1 in macrophages following HA treatment over 48 h was quantified using ELISA (n=3 for each group). (C) Western blotting analysis of p-STAT6 and total STAT6 expression at various time points following 100 μ g/ml HA stimulation (n=4 for each group). (D) Flow cytometric analysis of CD86 and CD206 expression in macrophages after 24-h treatment with 100 μ g/ml HA and CD44 inhibitor at indicated concentrations (n=3 for each group). (E) TGF-β1 quantification by ELISA in culture supernatants (n=3 for each group). (F) Western blotting analysis of p-STAT6 and total STAT6 in macrophages following 2-h treatment with HA and CD44 inhibitor at varying concentrations (n=3 for each group). (G) Western blotting analysis of p-STAT6 and total STAT6 in macrophages following 2-h treatment with HA and STAT6 inhibitor (AS1517499) at varying concentrations (n=4 for each group). (H) Schematic workflow: Conditioned medium from TGF-β1-stimulated NIH/3T3 fibroblasts was used to culture RAW264.7 macrophages for 24 h. Where indicated, RAW264.7 cells were co-treated with a CD44 inhibitor or a STAT6 inhibitor. Macrophage polarization was subsequently analyzed by flow cytometry. (I) Flow cytometry was used to measure the expression levels of CD206 (FITC) and CD86 (PE) (n=3 for each group). * P<0.05, ** P<0.01, *** P<0.001. HA, hyaluronic acid; FITC, fluorescein isothiocyanate; PE, phycoerythrin; p-, phosphorylated.
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    HA from myofibroblasts induces macrophage M2 polarization via the <t>CD44/STAT6</t> axis. (A) RAW264.7 macrophages were stimulated with HA for 24 h, and flow cytometry was performed to assess the expression of CD206 (FITC) and CD86 (PE) (n=3 for each group). (B) The content of TGF-β1 in macrophages following HA treatment over 48 h was quantified using ELISA (n=3 for each group). (C) Western blotting analysis of p-STAT6 and total STAT6 expression at various time points following 100 μ g/ml HA stimulation (n=4 for each group). (D) Flow cytometric analysis of CD86 and CD206 expression in macrophages after 24-h treatment with 100 μ g/ml HA and CD44 inhibitor at indicated concentrations (n=3 for each group). (E) TGF-β1 quantification by ELISA in culture supernatants (n=3 for each group). (F) Western blotting analysis of p-STAT6 and total STAT6 in macrophages following 2-h treatment with HA and CD44 inhibitor at varying concentrations (n=3 for each group). (G) Western blotting analysis of p-STAT6 and total STAT6 in macrophages following 2-h treatment with HA and STAT6 inhibitor (AS1517499) at varying concentrations (n=4 for each group). (H) Schematic workflow: Conditioned medium from TGF-β1-stimulated NIH/3T3 fibroblasts was used to culture RAW264.7 macrophages for 24 h. Where indicated, RAW264.7 cells were co-treated with a CD44 inhibitor or a STAT6 inhibitor. Macrophage polarization was subsequently analyzed by flow cytometry. (I) Flow cytometry was used to measure the expression levels of CD206 (FITC) and CD86 (PE) (n=3 for each group). * P<0.05, ** P<0.01, *** P<0.001. HA, hyaluronic acid; FITC, fluorescein isothiocyanate; PE, phycoerythrin; p-, phosphorylated.
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    Image Search Results


    HA from myofibroblasts induces macrophage M2 polarization via the CD44/STAT6 axis. (A) RAW264.7 macrophages were stimulated with HA for 24 h, and flow cytometry was performed to assess the expression of CD206 (FITC) and CD86 (PE) (n=3 for each group). (B) The content of TGF-β1 in macrophages following HA treatment over 48 h was quantified using ELISA (n=3 for each group). (C) Western blotting analysis of p-STAT6 and total STAT6 expression at various time points following 100 μ g/ml HA stimulation (n=4 for each group). (D) Flow cytometric analysis of CD86 and CD206 expression in macrophages after 24-h treatment with 100 μ g/ml HA and CD44 inhibitor at indicated concentrations (n=3 for each group). (E) TGF-β1 quantification by ELISA in culture supernatants (n=3 for each group). (F) Western blotting analysis of p-STAT6 and total STAT6 in macrophages following 2-h treatment with HA and CD44 inhibitor at varying concentrations (n=3 for each group). (G) Western blotting analysis of p-STAT6 and total STAT6 in macrophages following 2-h treatment with HA and STAT6 inhibitor (AS1517499) at varying concentrations (n=4 for each group). (H) Schematic workflow: Conditioned medium from TGF-β1-stimulated NIH/3T3 fibroblasts was used to culture RAW264.7 macrophages for 24 h. Where indicated, RAW264.7 cells were co-treated with a CD44 inhibitor or a STAT6 inhibitor. Macrophage polarization was subsequently analyzed by flow cytometry. (I) Flow cytometry was used to measure the expression levels of CD206 (FITC) and CD86 (PE) (n=3 for each group). * P<0.05, ** P<0.01, *** P<0.001. HA, hyaluronic acid; FITC, fluorescein isothiocyanate; PE, phycoerythrin; p-, phosphorylated.

    Journal: International Journal of Molecular Medicine

    Article Title: Orcinol glucoside ameliorates pulmonary fibrosis by suppressing hyaluronic acid synthesis and macrophage M2 polarization via targeting hyaluronic acid synthase 2

    doi: 10.3892/ijmm.2026.5764

    Figure Lengend Snippet: HA from myofibroblasts induces macrophage M2 polarization via the CD44/STAT6 axis. (A) RAW264.7 macrophages were stimulated with HA for 24 h, and flow cytometry was performed to assess the expression of CD206 (FITC) and CD86 (PE) (n=3 for each group). (B) The content of TGF-β1 in macrophages following HA treatment over 48 h was quantified using ELISA (n=3 for each group). (C) Western blotting analysis of p-STAT6 and total STAT6 expression at various time points following 100 μ g/ml HA stimulation (n=4 for each group). (D) Flow cytometric analysis of CD86 and CD206 expression in macrophages after 24-h treatment with 100 μ g/ml HA and CD44 inhibitor at indicated concentrations (n=3 for each group). (E) TGF-β1 quantification by ELISA in culture supernatants (n=3 for each group). (F) Western blotting analysis of p-STAT6 and total STAT6 in macrophages following 2-h treatment with HA and CD44 inhibitor at varying concentrations (n=3 for each group). (G) Western blotting analysis of p-STAT6 and total STAT6 in macrophages following 2-h treatment with HA and STAT6 inhibitor (AS1517499) at varying concentrations (n=4 for each group). (H) Schematic workflow: Conditioned medium from TGF-β1-stimulated NIH/3T3 fibroblasts was used to culture RAW264.7 macrophages for 24 h. Where indicated, RAW264.7 cells were co-treated with a CD44 inhibitor or a STAT6 inhibitor. Macrophage polarization was subsequently analyzed by flow cytometry. (I) Flow cytometry was used to measure the expression levels of CD206 (FITC) and CD86 (PE) (n=3 for each group). * P<0.05, ** P<0.01, *** P<0.001. HA, hyaluronic acid; FITC, fluorescein isothiocyanate; PE, phycoerythrin; p-, phosphorylated.

    Article Snippet: Antibodies for Collagen type I α 1 chain (COL1A1; cat. no. 72026; 1:1,000), Phospho-STAT6 (Tyr641; cat. no. 56554S; 1:1,000) and α-smooth muscle actin (α-SMA; cat. no. 19245; 1:1,000) were obtained from Cell Signaling Technology, Inc.

    Techniques: Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot